Related Experiment Video

Updated: Jan 2, 2026

Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae
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Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae

Published on: June 2, 2021

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MoFap7, a ribosome assembly factor, is required for fungal development and plant colonization of Magnaporthe oryzae

Lin Li1, Xue-Ming Zhu1, Huan-Bin Shi2

  • 1State Key Laboratory for Rice Biology, Biotechnology Institute, Zhejiang University, Hangzhou, China.

Virulence
|December 10, 2019
PubMed

Insights

MoFap7, a ribosome assembly factor in rice blast fungus, is crucial for fungal development and pathogenicity. Its ATPase activity and interactions regulate growth, infection, and stress response, offering insights into filamentous fungi.

Area of Science:

  • Molecular Biology
  • Mycology
  • Plant Pathology

Background:

  • Fap7 is a ribosome assembly factor essential for pre-40S subunit synthesis in yeast.
  • The functions of Fap7 homologs in filamentous fungi are largely unknown.
  • Magnaporthe oryzae causes rice blast disease, threatening global food security.

Purpose of the Study:

  • To identify and characterize the Fap7 homolog in M. oryzae (MoFap7).
  • To investigate the role of MoFap7 in fungal development, pathogenicity, and stress response.
  • To elucidate the molecular mechanisms underlying MoFap7 function in M. oryzae.

Main Methods:

  • Gene knockout (ΔMofap7) and site-directed mutagenesis.
  • Phenotypic analysis of mutants (growth, development, conidiation, appressorium formation, infection).
  • Biochemical assays (ATPase activity) and protein-protein interaction studies (co-immunoprecipitation).

Main Results:

  • ΔMofap7 mutants showed defects in growth, development, conidial morphology, appressorium formation, and infection.
  • Mutants were sensitive to oxidative stress, and conserved Walker A/B motifs were essential for MoFap7 function.
  • MoFap7 interacts with MoMst50 (MAPK Pmk1 pathway) and GTPases MoCdc42/MoRac1, regulating pathogenicity and growth.

Conclusions:

  • MoFap7 is essential for the development and pathogenicity of M. oryzae.
  • Its ATPase activity and interactions with signaling proteins are critical for orchestrating fungal processes.
  • This study provides novel insights into MoFap7's role in filamentous fungi.
Keywords:
MoFap7Pmk1 pathwayfungal developmentribosome assembly factorvirulence

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