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Multi-Photon Sensitized Excitation of Near Infrared Emitting Lanthanides
Grzegorz Piszczek1, Ignacy Gryczynski1, Badri P Maliwal1
1Center of Fluorescence Spectroscopy, University of Maryland at Baltimore, Department of Biochemistry and Molecular Biology, 725 West Lombard Street Baltimore, MD 21201.
Abstract:
Near infrared (NIR) multi-photon excitation of the NIR-emitting lanthanides neodymium (Nd3+) and ytterbium (Yb3+) sensitized by a fluorescein-linked chelator was demonstrated. Because tissues display minimal absorbance near the excitation wavelength of 800 nm, and because the lanthanides display long decay times, these results suggest the use of Nd3+ and Yb3+ as luminescent probes in tissues with multi-photon excitation.
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