A laccase based biosensor on AuNPs-MoS₂ modified glassy carbon electrode for catechol detection

Area of Science:

• Materials Science
• Nanotechnology
• Electrochemistry

Background:

• Two-dimensional layered nanomaterials like molybdenum disulfide (MoS2) nanosheets are increasingly utilized in biosensor applications.
• MoS2 nanosheets offer a large specific surface area and excellent biocompatibility, crucial for effective enzyme immobilization.

Purpose of the Study:

• To develop a novel laccase-based biosensor for sensitive and selective catechol detection.
• To investigate the synergistic effects of MoS2 nanosheets and gold nanoparticles in enhancing biosensor performance.

Main Methods:

• Fabrication of a nanocomposite material using MoS2 nanosheets and gold nanoparticles.
• Immobilization of laccase enzyme onto the MoS2-gold nanoparticle nanocomposite.
• Electrochemical characterization and performance evaluation of the fabricated biosensor for catechol detection.

Main Results:

• The MoS2-gold nanoparticle nanocomposite provided abundant sites for enzyme immobilization and enhanced conductivity.
• The laccase-based biosensor demonstrated a linear response to catechol over a wide concentration range (2–2000 μM).
• A low detection limit of 2 μM (S/N=3) was achieved, along with excellent selectivity, stability, repeatability, and reproducibility.

Conclusions:

• The synergistic combination of MoS2 nanosheets and gold nanoparticles significantly improves the performance of laccase-based biosensors.
• The developed biosensor is a promising tool for accurate and reliable catechol detection.