A laccase based biosensor on AuNPs-MoS₂ modified glassy carbon electrode for catechol detection

As a kind of two-dimensional layered nanomaterial, MoS₂ nanosheets have been widely used in the field of biosensors. In this work, a novel laccase based biosensor for catechol detection was fabricated based on a nanocomposite of MoS₂ nanosheets and gold nanoparticles. The experimental results demonstrated that MoS₂ had large specific surface area and good biocompatibility, which provided abundant position for enzyme immobilization. Gold nanoparticles enhanced conductivity of MoS₂ and improved detection sensitivity. Because of the synergistic effect of MoS₂ nanosheets and gold nanoparticles, the laccase based bioelectrode exhibited a linear response to catechol from 2 to 2000 μM with a detection limit of 2 μM (S/N = 3), as well as good selectivity, stability, repeatability, and reproducibility.