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Soft glass multi-channel capillaries as a platform for bioimprinting
Natalia A Burmistrova1, Pavel S Pidenko1, Sergey A Pidenko1
1Institute of Chemistry, Saratov State University, Astrakhanskaya 83, 410012, Saratov, Russia.
Talanta
|December 11, 2019
Summary
This study introduces a novel immunoassay using imprinted proteins on multi-channel capillaries for sensitive detection of food contaminants like zearalenone. The in-situ imprinting method achieved a low limit of detection, offering a promising alternative to existing screening tests.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Materials Science
Background:
- Multi-channel capillaries (MC) offer large surface area and stability for sensor applications.
- Molecular imprinting provides selective recognition elements for bioassays.
- Existing screening tests for food contaminants can be improved for sensitivity and cost-effectiveness.
Purpose of the Study:
- To develop a novel immunoassay format using imprinted proteins on MC platforms.
- To compare two bioimprinting approaches: pre-immobilized vs. in-situ imprinting.
- To demonstrate the assay's efficacy in detecting the food contaminant zearalenone (ZEN).
Main Methods:
- Modification of MC inner surfaces with poly-l-lysine.
- Preparation and immobilization of imprinted ovalbumin (OVA) on MC.
- Comparison of pre-prepared imprinted OVA immobilization versus in-situ imprinting directly on MC.
- Detection of zearalenone (ZEN) using the developed bioimprinted assay.
- Validation in artificially spiked wheat samples.
Main Results:
- In-situ imprinted OVA on MC achieved a limit of detection (LOD) of 0.12 ng/mL for ZEN.
- Pre-immobilized imprinted OVA on MC yielded an LOD of 0.8 ng/mL.
- The assay sensitivity was comparable to commercial ELISA kits for ZEN detection.
- Testing in spiked wheat samples showed high recovery (88-112%) and good repeatability (8.5-9.6% RSD).
Conclusions:
- In-situ imprinting of proteins on MC surfaces is a highly effective strategy for enhancing assay sensitivity.
- The developed imprinted protein (IP)-based MC-ELISA is a promising tool for mycotoxin analysis in complex food matrices.
- This technique offers an environmentally friendly, cost-effective, and sensitive alternative for food safety screening.
