Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.5K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.5K
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.4K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.4K
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

998
When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
998
Chemical Shift: Internal References and Solvent Effects01:17

Chemical Shift: Internal References and Solvent Effects

1.2K
In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
1.2K
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

572
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
572
¹H NMR Signal Integration: Overview00:58

¹H NMR Signal Integration: Overview

3.2K
The intensity of a signal, which can be represented by the area under the peak, depends on the number of protons contributing to that signal. The area under each peak is shown as a vertical line called an integral, with the integral value listed under it, as seen in the proton NMR spectrum of benzyl acetate. Each integral value is divided by the smallest integral value to obtain the ratio of the number of protons producing each signal. The ratio reveals the relative number of protons and not...
3.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dietary Patterns Following an Episode of Acute Pancreatitis: A Post Hoc Analysis From the PAPPEI Multicenter, Prospective Study.

Pancreas·2026
Same author

Metabolite names and identifiers: how far are we from interoperability?

Metabolomics : Official journal of the Metabolomic Society·2026
Same author

Guideline organizations' guidance documents paper 5: conflict of interest and funding.

Journal of clinical epidemiology·2025
Same author

<sup>13</sup>C position-specific isotopic analysis of fatty acid methyl esters using NMR with intramolecular isotopic referencing.

Analytical and bioanalytical chemistry·2025
Same author

Is it possible to distinguish virgin <i>versus</i> pyrolytic recycled styrene by determining the intramolecular <sup>13</sup>C distribution?

Analytical methods : advancing methods and applications·2025
Same author

WEST: an "all-terrain" multiple signal suppression technique for quantitative <sup>1</sup>H NMR.

Analytica chimica acta·2025

Related Experiment Video

Updated: Jan 2, 2026

NMR Spectroscopy as a Robust Tool for the Rapid Evaluation of the Lipid Profile of Fish Oil Supplements
08:54

NMR Spectroscopy as a Robust Tool for the Rapid Evaluation of the Lipid Profile of Fish Oil Supplements

Published on: May 1, 2017

26.7K

Improved lipid mixtures profiling by 1H NMR using reference lineshape adjustment and deconvolution techniques.

Ghina Hajjar1, Noelle Merchak1, Charbel Daniel2

  • 1Laboratory of Metrology and Isotopic Fractionation, Research Unit: Technologies et Valorisation Agroalimentaire (TVA), Faculty of Science, Saint Joseph University of Beirut, P.O. Box 17-5208 Mar Mikhael, Beirut, 1104 2020, Lebanon; EBSI Team, Interdisciplinary Chemistry: Synthesis, Analysis, Modelling (CEISAM), University of Nantes - CNRS UMR 6230, 2 rue de la Houssinière, BP 92208, F-44322, Nantes Cedex 3, France.

Talanta
|December 11, 2019
PubMed
Summary

This study introduces a novel quantitative approach for analyzing complex mixtures using 1D proton NMR spectroscopy. The method enhances spectral deconvolution, improving the precision and accuracy of lipid quantitation in natural products like olive oil.

Keywords:
(1)H NMR spectral deconvolutionFatty acids quantitationMultivariate modelsOlive oil authenticationReference lineshape adjustmentTriacylglycerols

More Related Videos

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
09:38

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

9.1K
Lipidomics and Transcriptomics in Neurological Diseases
09:58

Lipidomics and Transcriptomics in Neurological Diseases

Published on: March 18, 2022

3.9K

Related Experiment Videos

Last Updated: Jan 2, 2026

NMR Spectroscopy as a Robust Tool for the Rapid Evaluation of the Lipid Profile of Fish Oil Supplements
08:54

NMR Spectroscopy as a Robust Tool for the Rapid Evaluation of the Lipid Profile of Fish Oil Supplements

Published on: May 1, 2017

26.7K
Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
09:38

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

9.1K
Lipidomics and Transcriptomics in Neurological Diseases
09:58

Lipidomics and Transcriptomics in Neurological Diseases

Published on: March 18, 2022

3.9K

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Chemometrics

Background:

  • Proton nuclear magnetic resonance (¹H NMR) spectroscopy is valuable for analyzing complex mixtures like natural product lipids.
  • However, signal overlap and spectral distortions in ¹H NMR spectra hinder accurate quantitation.
  • Traditional spectral integration methods provide limited quantitative information due to signal overlap.

Purpose of the Study:

  • To develop and validate a robust quantitation method for complex ¹H NMR spectra.
  • To improve the precision and accuracy of lipid quantitation in natural extracts.
  • To enhance the classification and authentication of complex mixtures using NMR data.

Main Methods:

  • A novel quantitation approach based on spectral deconvolution using reference lineshape adjustment (RLA) was developed.
  • The RLA method iteratively refines spectral fit and peak precision.
  • The approach was applied to ¹H NMR spectra of olive oil samples.

Main Results:

  • The RLA-based deconvolution extracted significantly more spectral variables (77 peaks) compared to traditional integration (5-29 variables).
  • Deconvoluted peak intensities and areas showed improved precision after RLA.
  • The method enabled accurate quantitation of specific fatty acids (oleic, palmitoleic, vaccenic, linoleic, linolenic) in olive oil, which was previously challenging or impossible.

Conclusions:

  • The RLA-based deconvolution approach offers a powerful tool for precise quantitation of complex ¹H NMR spectra.
  • This method significantly enhances the characterization and authentication of natural products like olive oil.
  • The improved spectral data facilitates more accurate multivariate statistical analysis for sample classification.