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'Artificial peroxidase' nanozyme - enzyme based lactate biosensor
Darya V Vokhmyanina1, Ksenia D Andreeva1, Maria A Komkova1
1Chemistry Faculty of M.V. Lomonosov Moscow State University, 119991, Moscow, Russia.
Talanta
|December 11, 2019
Summary
This study introduces a novel nanozyme-enzyme biosensor for lactate detection, replacing traditional enzymes with highly active and stable "artificial peroxidase" nanoparticles. This innovation significantly enhances sensitivity and operating stability for improved biosensing applications.
Area of Science:
- Biotechnology
- Nanotechnology
- Electrochemistry
Background:
- Bienzyme biosensors often face limitations in stability and activity.
- Peroxidase enzymes can be substituted with more robust nanomaterials.
- Hydrogen peroxide transducers are crucial components in many biosensors.
Purpose of the Study:
- To develop a highly sensitive and stable nanozyme-enzyme based biosensor for lactate detection.
- To utilize Prussian Blue nanozymes as an
Main Methods:
- Developed a nanozyme-enzyme biosensor by replacing peroxidase with Prussian Blue nanozymes.
- Employed composite alkoxysilane-perfluorosulfonated ionomer (PFSI) membranes for lactate oxidase immobilization.
- Investigated the sensitivity and operating stability of the developed biosensor.
Main Results:
- The nanozyme-enzyme biosensor demonstrated significantly higher sensitivity (>0.2 A M-1 cm-2) compared to traditional Prussian Blue film biosensors.
- The use of PFSI membranes enhanced the operating stability of the lactate oxidase immobilization.
- Catalytically synthesized Prussian Blue nanozymes simplified the hydrogen peroxide transducer assembly, increasing sensitivity.
Conclusions:
- Nanozyme-artificial peroxidase offers a promising alternative to traditional enzymes in biosensor development.
- The developed nanozyme-enzyme biosensor exhibits superior performance for lactate detection.
- This approach is expected to be widely applicable for various oxidase-based biosensors.
