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Multi-Electrode Array with a Planar Surface for Cell Patterning by Microprinting.

Jan Slavík1, Josef Skopalík2, Ivo Provazník2,3

  • 1Central European Institute of Technology, Brno University of Technology, Purkyňova 123, 616 00 Brno, Czech Republic.

Sensors (Basel, Switzerland)
|December 11, 2019
PubMed
Summary

A new fabrication method created planar multielectrode arrays (pMEAs) for uniform cell contact. These pMEAs offer comparable impedance to conventional devices and enable precise cell patterning for electrophysiological studies.

Keywords:
HL-1 cellsantifouling agentmicroelectrode arraymicroprintingmultielectrode arraypeel-offsacrificial layer

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Area of Science:

  • Biomedical Engineering
  • Neuroscience
  • Materials Science

Background:

  • Multielectrode arrays (MEAs) are crucial for non-invasive electrophysiological measurements of cell populations.
  • Conventional MEAs often have recessed electrodes, limiting uniform surface contact.
  • Achieving consistent cell adhesion and patterning on MEAs is essential for reliable recordings.

Purpose of the Study:

  • To introduce a novel fabrication method for planar multielectrode arrays (pMEAs).
  • To demonstrate the advantages of pMEAs for uniform cell contact and patterning.
  • To characterize the electrophysiological properties of pMEAs.

Main Methods:

  • Fabrication of pMEAs using a low adhesive gold sacrificial peel-off layer.
  • Characterization of the insulation layer profile and planar electrode impedance.
  • Testing pMEA performance for HL-1 cell patterning using fibronectin and PLL-g-PEG coating.

Main Results:

  • The pMEA fabrication resulted in a fully planar surface with electrodes and insulation layer on the same plane.
  • pMEA impedance was comparable to conventional MEA electrodes.
  • Successful patterning of HL-1 cells was achieved, maintaining pattern integrity at confluency.
  • Patterned HL-1 cells exhibited spontaneous and synchronous beating activity.

Conclusions:

  • The novel pMEA design offers a planar surface ideal for microfluidic integration and cell patterning.
  • pMEAs provide a viable alternative to conventional MEAs with comparable electrical performance.
  • This technology facilitates advanced cellular electrophysiology studies with improved cell adhesion and patterning.