Comparative genomics analysis of Nitriliruptoria reveals the genomic differences and salt adaptation strategies

The group Nitriliruptoria, recently classified as a separate class of phylum Actinobacteria, has five members at present, which belong to halophilic or halotolerant Actinobacteria. Here, we sequenced the genomes of Egicoccus halophilus EGI 80432^T and Egibacter rhizosphaerae EGI 80759^T, and performed a comparative genomics approach to analyze the genomic differences and salt adaptation mechanisms in Nitriliruptoria. Phylogenetic analysis suggested that Euzebya tangerina F10^T has a closer phylogenetic relationship to Euzebya rosea DSW09^T, while genomic analysis revealed highest genomic similarity with Nitriliruptor alkaliphilus ANL-iso2^T and E. halophilus EGI 80432^T. Genomic differences of Nitriliruptoria were mainly observed in genome size, gene contents, and the amounts of gene in per functional categories. Furthermore, our analysis also revealed that Nitriliruptoria possess similar synthesis systems of solutes, such as trehalose, glutamine, glutamate, and proline. On the other hand, each member of Nitriliruptoria species possesses specific mechanisms, K⁺ influx and efflux, betaine and ectoine synthesis, and compatible solutes transport to survive in various high-salt environments.