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Updated: Jan 2, 2026

Rare Event Detection Using Error-corrected DNA and RNA Sequencing
Published on: August 3, 2018
Development of microsatellite markers for the threatened species Coleocephalocereus purpureus (Cactaceae) using
Daphne Amaral Fraga1, Anderson Figueiredo de Carvalho1, Ricardo Souza Santana2
1Molecular Biology Lab, Department of Science and Languages, Federal Institute of Minas Gerais, Bambuí Campus, Bambuí, Minas Gerais State, Brazil.
Abstract:
Ten microsatellite loci were developed and validated for the endangered cactus species Coleocephalocereus purpureus. The markers were obtained from sequences generated by whole genome shotgun sequencing approaches. A testing group of 36 specimens of the main population were genotyped and all described markers presented suitable outcomes to population genetic studies, showing polymorphic status for C. purpureus testing group with clean and reproducible amplification. No evidence for scoring errors, null alleles or linkage disequilibrium was detected. Number of alleles per locus ranged from 3 to 6 and expected heterozygosity ranged from 0.78 to 0.99. These new microsatellite loci are suitable to be used in future diversity and structure population studies of C. purpureus.

