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Principles Of Column Chromatography01:13

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The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
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Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
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In chromatography, a solute moves through a chromatographic column and tends to spread, forming a Gaussian-shaped band. The longer the solute spends in the column, the broader the band becomes. The broadening can lead to overlaps within the column, affecting separation effectiveness.
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High-Performance Liquid Chromatography: Introduction01:11

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Size-Exclusion Chromatography01:08

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In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
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Tritium-Labeled Compounds VI. Alditols-<i>1-t</i> and Alditols-<i>2-t</i>.

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Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
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Large-Scale, Preparative Paper Chromatography.

Harriet L Frush1

  • 1Institute for Materials Research, National Bureau of Standards, Washington, D.C. 20234.

Journal of Research of the National Bureau of Standards. Section A, Physics and Chemistry
|December 12, 2019
PubMed
Summary
This summary is machine-generated.

This study introduces simplified techniques for large-scale preparative chromatography using heavy papers. A novel method for non-destructively locating compound bands on wet chromatograms is also presented.

Keywords:
Chromatography on seed-test paperlarge-scale paper chromatographyradioactive carbohydratesuse of heavy papers in preparative chromatography

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Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Large-scale preparative chromatography requires efficient and scalable methods.
  • Accurate detection of separated compounds on chromatograms is crucial for analysis.

Purpose of the Study:

  • To describe simplified techniques for using heavy papers in large-scale preparative chromatography.
  • To present a new method for non-destructively locating compound bands on wet chromatograms.
  • To illustrate the capacities and behavior of specific heavy papers.

Main Methods:

  • Utilized simplified techniques for preparative chromatography with heavy papers.
  • Developed and demonstrated a novel technique for non-destructive band detection on wet chromatograms.
  • Evaluated the performance characteristics of Whatman seed-test paper and Whatman No. 17 paper.

Main Results:

  • Established simplified methods for employing heavy papers in preparative chromatography.
  • Successfully demonstrated a new technique for non-destructive visualization of compound bands.
  • Characterized the capacities and behavior of Whatman seed-test and Whatman No. 17 papers.

Conclusions:

  • Simplified techniques enhance the utility of heavy papers for large-scale preparative chromatography.
  • The new non-destructive detection method improves chromatogram analysis.
  • Whatman seed-test and Whatman No. 17 papers exhibit specific, illustrated characteristics relevant to preparative applications.