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Related Experiment Video

Updated: Jan 2, 2026

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A large size-selective DNA nanopore with sensing applications.

Rasmus P Thomsen1, Mette Galsgaard Malle2,3, Anders Hauge Okholm1,4

  • 1Interdisciplinary Nanoscience Center, Aarhus University, Aarhus C, 8000, Denmark.

Nature Communications
|December 13, 2019
PubMed
Summary

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Researchers engineered a synthetic DNA nanopore for controlled molecular transport across lipid bilayers. This programmable pore enables selective cargo translocation and real-time signal detection for advanced sensing applications.

Area of Science:

  • Nanotechnology
  • Biophysics
  • Synthetic Biology

Background:

  • Transmembrane nanostructures, such as ion channels, regulate molecular flow across lipid bilayers.
  • Engineered artificial nanopores show promise for selective gating, label-free biomolecule detection, and DNA sequencing.
  • Developing synthetic nanopores with controlled functions is crucial for advancing molecular transport and sensing technologies.

Purpose of the Study:

  • To engineer a synthetic DNA nanopore using DNA origami with programmable control and size-selective gating.
  • To validate the assembly and lipid bilayer integration of the DNA nanopore.
  • To demonstrate the nanopore's capability for selective cargo translocation and real-time signal detection.

Main Methods:

  • DNA origami was used to construct a 9 nm wide synthetic DNA nanopore with lipidated flaps.

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  • Transmission electron microscopy (TEM) confirmed nanopore assembly and insertion into lipid bilayers.
  • Optical methods, including single-molecule total internal reflection fluorescence (TIRF) microscopy, were employed to study cargo translocation and pore mechanosensitivity.

    • Main Results:

    • Successful assembly and insertion of the DNA nanopore into lipid bilayers were validated.
    • Selective translocation of macromolecules through the nanopore was demonstrated.
    • Oligonucleotide-triggered pore opening and size-specific cargo transport confirmed its function as a real-time detection system.

    Conclusions:

    • The synthetic DNA nanopore functions as a programmable gate for controlled macromolecule translocation.
    • The nanopore's responsiveness to external signals, like oligonucleotides, enables real-time detection capabilities.
    • This engineered DNA nanopore holds potential for highly parallelized sensing applications in various biological contexts.