Related Experiment Video
Updated: Jul 21, 2026
07:48
An Orthotopic Bladder Cancer Model for Gene Delivery Studies
Published on: December 1, 2013
13.0K
Bioinformatics Analysis Identified Key Molecular Changes in Bladder Cancer Development and Recurrence.
Qingke Chen1, Jieping Hu1, Jun Deng1
1Department of Urology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, China.
Biomed Research International
|December 13, 2019
Summary
Key genes like CCNB1, ESPL1, CENPM, BLM, and ASPM are significantly upregulated in bladder cancer (BC), indicating their potential roles in BC development and recurrence. This study analyzed gene expression to identify these critical molecular changes.
Area of Science:
- Oncology
- Genomics
- Molecular Biology
Background:
- Bladder cancer (BC) presents significant challenges due to high recurrence and mortality rates.
- Understanding the molecular underpinnings of BC is crucial for developing effective treatments.
Purpose of the Study:
- To identify key molecular alterations and potential biomarkers in bladder cancer.
- To analyze gene expression profiles and their correlation with clinical outcomes.
Main Methods:
- Analysis of next-generation sequencing data from clinical bladder cancer specimens.
- Utilized DAVID for Gene Ontology (GO) and KEGG pathway enrichment analyses.
- Identified hub genes using MCODE in Cytoscape and analyzed protein expression and survival data from OncoLnc and ONCOMINE.
Main Results:
- Identified 4211 differentially expressed genes (DEGs) between cancer and surrounding tissues, and 410 DEGs between cancer and recurrent tissues.
- GO analysis highlighted enrichment in cytoplasmic and nucleoplasmic genes; KEGG analysis revealed enrichment in the PI3K-Akt signaling pathway.
- CCNB1, ESPL1, CENPM, BLM, and ASPM were identified as hub genes associated with overall survival, showing significantly higher expression in BC tissues.
Conclusions:
- CCNB1, ESPL1, CENPM, BLM, and ASPM are implicated in bladder cancer development.
- CCNB1, ESPL1, CENPM, and BLM may also play a role in bladder cancer recurrence.
