Polysaccharide from Caulerpa lentillifera: extraction optimization with response surface methodology, structure and
Hua Tian1, Haifang Liu2, Weikang Song1
1Hainan Provincial Fine Chemical Engineering Research Center, Hainan University, Haikou, P.R. China.
Abstract:
Ultrasonic-assisted extraction based on Response Surface Methodology was applied to isolate a polysaccharide (CLP) from Caulerpa lentillifera obtained in the South China Sea and the highest yield of the crude polysaccharide was 11.8%. CLP consisted of xylose, galactose, glucose and glucuronic acid in a molar ratio of 2.00: 1.00: 0.26: 0.04, with a molecular weight of 115 kDa. The content of uronic acids and sulfate groups in CLP was 14.10% and 27.40%, respectively. CLP presented good radical scavenging activities against O2·-, DPPH radical, while the scavenging activities against OH· and ABTS radical were not so satisfying.
