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Complete Genome Sequence of Citrobacter freundii Siphophage Sazh

Whitney L Crossland1, Justin P Shaw1, Chandler O'Leary1

  • 1Center for Phage Technology, Texas A&M University, College Station, Texas, USA.

Microbiology Resource Announcements
|December 14, 2019
PubMed

Insights

The complete genome sequence of Citrobacter freundii siphophage Sazh was determined. This bacteriophage may be useful for developing new therapies against multidrug-resistant Citrobacter freundii infections.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Citrobacter freundii is an opportunistic pathogen causing various nosocomial infections.
  • Multidrug-resistant strains of C. freundii pose a significant therapeutic challenge.
  • Bacteriophages (phages) are viruses that infect bacteria and are being explored as antimicrobial agents.

Purpose of the Study:

  • To determine the complete genome sequence of Citrobacter freundii siphophage Sazh.
  • To analyze the genomic features of Sazh and its phylogenetic relationship to other bacteriophages.

Main Methods:

  • Whole-genome sequencing of phage Sazh.
  • Bioinformatic analysis of the Sazh genome.
  • Comparative genomics with related bacteriophages.

Main Results:

  • The complete genome sequence of C. freundii siphophage Sazh was successfully obtained.
  • Sazh shares close evolutionary relationships with Enterobacteria phages T1 and TLS.
  • Genomic analysis provides insights into the Sazh phage's structure and potential lytic mechanisms.

Conclusions:

  • The genomic data of Sazh provides a foundation for its potential application as a therapeutic agent.
  • Understanding phage-host interactions is crucial for developing phage therapy against C. freundii infections.
  • Further research into Sazh's efficacy and safety is warranted for clinical applications.

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