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Published on: October 24, 2016
Optimization of pre-saccharification time during dSSF process in oat-hull bioethanol technology
Galina F Mironova1, Ekaterina A Skiba1, Aleksey A Kukhlenko1
1Laboratory of Bioconversion, Laboratory of Chemical Engineering Processes and Apparatuses, Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences (IPCET SB RAS), Biysk, 659322 Altai Krai Russia.
Optimizing pre-saccharification time for oat hull pulp in simultaneous saccharification and fermentation with delayed yeast inoculation (dSSF) enhances bioethanol yield. A 24-hour pre-saccharification period proved optimal for maximum cellulose and hemicellulose hydrolysis.
Area of Science:
- Biotechnology
- Biochemical Engineering
- Renewable Energy
Background:
- Lignocellulosic biomass is a sustainable feedstock for bioethanol production.
- Simultaneous saccharification and fermentation (SSF) processes require optimization for efficient conversion.
- Delayed yeast inoculation (dSSF) can improve process control and yield.
Purpose of the Study:
- To mathematically describe and optimize the pre-saccharification time in dSSF for bioethanol production.
- To determine the optimal pre-saccharification duration for alkaline-delignified oat hull pulp.
- To maximize cellulose and hemicellulose hydrolysis and subsequent bioethanol yield.
Main Methods:
- Utilized alkaline-delignified oat hull pulp as a substrate.
- Performed pre-saccharification at 60 g/L solid loading and 46°C with mixed enzymes (CelloLux-A, BrewZyme BGX) for varying durations (8-72 h).
- Inoculated with Saccharomyces cerevisiae Y-1693 at 28°C for combined saccharification and fermentation.
Main Results:
- Identified an optimal pre-saccharification time of 24 hours for the tested conditions.
- Achieved maximum hydrolysis of cellulose and hemicelluloses at the 24-hour mark.
- The 24-hour pre-saccharification led to the highest bioethanol yield.
Conclusions:
- A 24-hour pre-saccharification step is optimal for the dSSF of oat hull pulp, maximizing bioethanol production.
- The developed method for optimizing pre-saccharification time is applicable to other lignocellulosic feedstocks.
- This optimization strategy contributes to more efficient and sustainable bioethanol manufacturing.
