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Updated: Jan 1, 2026
Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury
Published on: March 7, 2022
LncRNA MALAT1 knockdown alleviates myocardial apoptosis in rats with myocardial ischemia-reperfusion through
1Division of Cardiology, Anzhen Hospital Capital Medical University, Beijing, China. hjzhang73@126.com.
Objective:
To observe the effect of long non-coding ribonucleic acid metastasis-associated lung adenocarcinoma transcript 1 (lncRNA MALAT1) on the myocardial ischemia-reperfusion (I/R) injury in rats and to explore its potential mechanism, to provide certain references for clinical prevention and treatment of myocardial I/R injury.
Materials And Methods:
A total of 60 male Wistar rats were randomly divided into the Control group (n=20), I/R group (n=20) and I/R + MALAT1 small-interfering RNA (siRNA) group (n=20) using a random number table. The I/R model was established through recanalization after ligation of left anterior descending coronary artery (LAD), and the MALAT1 knockdown model was established via tail intravenous injection of MALAT1 siRNA in the I/R + MALAT1 siRNA group. The ejection fraction (EF%) and fractional shortening (FS%) of rats in each group were detected via echocardiography and the infarction area in each group was detected using 2,3,5-triphenyl tetrazolium chloride (TTC) assay. Moreover, the morphological changes in myocardial cells in each group were detected via hematoxylin-eosin (H&E) staining, and the myocardial apoptosis level was detected via terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining. At the same time, the expression levels of the anti-apoptotic protein B-cell lymphoma-2 (Bcl-2) and pro-apoptotic protein Bcl-2 associated X protein (Bax) in myocardial tissues in each group were determined via Western blotting. Finally, the effect of MALAT1 knockdown on the phosphatidylinositol 3-hydroxy kinase/protein kinase B (PI3K/AKT) protein expression was detected via Western blotting.
Results:
The expression level of lncRNA MALAT1 in myocardial tissues was significantly higher in the I/R group than that in the Control group (p<0.05). The MALAT1 knockdown could significantly improve the cardiac insufficiency caused by I/R injury, and increase both EF% and FS% in rats (p<0.05). In addition, the MALAT1 knockdown could markedly inhibit myocardial infarction caused by I/R injury and reduce the infarction area from (62.12 ± 1.29) to (27.66 ± 3.58; p<0.05). The results of the H&E staining showed that the myofilaments were arranged more orderly, the degrees of degradation and necrosis were lower and the cellular edema was significantly alleviated in the I/R + MALAT1 siRNA group compared with those in the I/R group. According to the results of TUNEL staining, the rats in I/R + MALAT1 siRNA group had a markedly lower level of myocardial apoptosis than the I/R group (p<0.05), and the Bax/Bcl-2 ratio also remarkably declined in the I/R + MALAT1 siRNA group (p<0.05). Furthermore, the results of Western blotting revealed that MALAT1 siRNA could significantly reverse the I/R injury-induced inhibition on the AKT phosphorylation (p<0.05).
Conclusions:
The MALAT1 knockdown can markedly improve the I/R-induced myocardial injury and promote the cardiac function of rats, whose mechanism may be related to the activation of the AKT signaling pathway by MALAT1 siRNA. Therefore, lncRNA MALAT1 is expected to be a new therapeutic target for myocardial I/R injury.
Insights
Knocking down long non-coding RNA MALAT1 significantly improves heart function and reduces injury in rats following myocardial ischemia-reperfusion. This protective effect is linked to the activation of the AKT signaling pathway, suggesting MALAT1 as a potential therapeutic target.
Area of Science:
- Molecular Biology
- Cardiovascular Research
- Biochemistry
Background:
- Myocardial ischemia-reperfusion (I/R) injury is a critical condition in cardiovascular disease.
- Long non-coding RNAs (lncRNAs) play emerging roles in cellular processes, including injury responses.
- Metastasis-associated lung adenocarcinoma transcript 1 (lncRNA MALAT1) has been implicated in various pathologies.
Purpose of the Study:
- To investigate the effect of lncRNA MALAT1 on myocardial I/R injury in a rat model.
- To explore the underlying molecular mechanisms, particularly the involvement of the PI3K/AKT pathway.
- To assess the therapeutic potential of targeting lncRNA MALAT1 for myocardial I/R injury.
Main Methods:
- Establishment of a rat model of myocardial I/R injury.
- lncRNA MALAT1 knockdown using small-interfering RNA (siRNA) via tail vein injection.
- Assessment of cardiac function (echocardiography), infarct size (TTC staining), myocardial cell morphology (H&E staining), apoptosis (TUNEL staining), and protein expression (Western blotting for Bcl-2, Bax, and AKT phosphorylation).
Main Results:
- lncRNA MALAT1 expression was significantly upregulated in the I/R group.
- MALAT1 knockdown improved cardiac function (increased EF% and FS%), reduced infarct size, and alleviated myocardial cell damage and apoptosis.
- MALAT1 knockdown reversed the inhibition of AKT phosphorylation induced by I/R injury.
Conclusions:
- lncRNA MALAT1 knockdown significantly mitigates myocardial I/R injury in rats.
- The protective mechanism involves the activation of the AKT signaling pathway.
- lncRNA MALAT1 represents a promising therapeutic target for managing myocardial I/R injury.
