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Mary K Burdette1,2, Ragini Jenkins1,2, Yuriy P Bandera1,2
1Department of Materials Science and Engineering, Advanced Materials Research Laboratories, Clemson University, Anderson, South Carolina 29625, United States.
ACS Applied Bio Materials
|December 18, 2019
Summary
This study introduces a novel polymer nanoparticle platform for personalized nanotheranostics, combining photodynamic therapy and active targeting. The system demonstrates controlled payload release and enhanced cancer cell death, paving the way for advanced targeted therapies.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Cancer Therapy
Background:
- Personalized nanotheranostics require platforms for simultaneous diagnosis and therapy.
- Current nanocarriers face challenges with payload release and targeting efficiency.
- Developing stimuli-responsive systems is crucial for controlled drug delivery.
Purpose of the Study:
- To develop a multifunctional polymer nanoparticle platform for personalized nanotheranostic applications.
- To enable controlled photodynamic therapy and active targeting.
- To create a system with environmentally-triggered payload release and signal activation.
Main Methods:
- Surface modification of poly(propargyl acrylate) (PA) nanoparticles with azide-modified bovine serum albumin (azBSA) as a linker.
- Attachment of organic ligands and fluorophores (payload) via azBSA for environmentally-sensitive release.
- Utilized human head and neck squamous carcinoma cells (UMSCC22A) for photodynamic therapy studies.
- Employed human lung carcinoma cells (A549) for toxicity studies with Survivin-targeted nanoparticles.
Main Results:
- Photodynamic therapy with the nanoparticles showed significant reduction in UMSCC22A cell growth.
- Payload emission was quenched upon attachment and reactivated upon azBSA digestion, improving signal-to-noise ratio.
- The system demonstrated versatility with different fluorophores (silicon phthalocyanine, cyanine 3).
- Survivin-targeted nanoparticles enhanced cell death in A549 cells compared to free targeting ligand.
Conclusions:
- The developed polymer nanoparticle platform is a versatile tool for personalized nanotheranostics.
- The system allows for controlled payload release and targeted delivery, enhancing therapeutic efficacy.
- This platform shows promise for advanced cancer treatment strategies combining photodynamic therapy and active targeting.
