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Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
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Analytical techniques for metabolomic studies: a review.

Karen Segers1,2, Sven Declerck1, Debby Mangelings1

  • 1Department of Analytical Chemistry, Applied Chemometrics & Molecular Modelling (FABI), Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090 Brussels, Belgium.

Bioanalysis
|December 18, 2019
PubMed
Summary

Metabolomics studies small molecules using techniques like NMR and MS. This review covers common analytical methods, their pros and cons, and applications for comprehensive metabolome analysis.

Keywords:
multiplatform approachesseparation techniquesspectrometric techniquesspectroscopic techniquestargeted metabolomicsuntargeted metabolomics

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Area of Science:

  • Metabolomics and analytical chemistry.

Background:

  • Metabolomics involves the comprehensive study of small-molecule metabolites.
  • Achieving broad metabolome coverage is difficult due to diverse physicochemical properties of metabolites.

Purpose of the Study:

  • To review commonly used analytical techniques in metabolomic studies.
  • To discuss the advantages, drawbacks, and applications of these techniques.

Main Methods:

  • Spectroscopic techniques (NMR).
  • Spectrometric techniques (MS).
  • Separation techniques including LC, GC, supercritical fluid chromatography, and CE.

Main Results:

  • The choice of analytical technique depends on sample matrix, metabolite properties, and sample amount.
  • Each technique offers different coverage and is suited for specific metabolite types.

Conclusions:

  • Understanding the strengths and limitations of various analytical techniques is crucial for effective metabolomic research.
  • Selecting appropriate methods ensures comprehensive and accurate metabolome profiling.