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Updated: Jan 1, 2026
Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins
Published on: December 27, 2016
Reversible detection of phosphorylation and dephosphorylation by tip-enhanced Raman spectroscopy using a
Yi-Cheng Lin1, Zi-Yu Ke, Pei-Hsuan Liao
1Department of Chemistry, National Taiwan University, No. 1, Section 4, Roosevelt Road, Taipei 10617, Taiwan. kvkong@ntu.edu.tw.
Abstract:
The detection of cancer invasion is crucial for diagnosis. In this report, we employed a TERS tip and SERS nanotags to create a cell signaling based nano-sensing system. This system is capable of creating a reversible phosphorylation/de-phosphorylation cycle for TERS measurement. The reversible TERS sensing is then paired with a downstream binding domain, Src homology region 2 (SH2), which is associated with the cell signaling for cancer cell invasion. Such a system offers the advantages of convenient detection of nanotags and high sensitivity as validated in a cell model.
