Related Experiment Video

Updated: Jan 1, 2026

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation
08:07

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation

Published on: September 6, 2017

10.5K

Donor selection in a pediatric stem cell transplantation cohort using PIRCHE and HLA-DPB1 typing

Wiebke Stenger1, Annette Künkele1,2,3,4, Matthias Niemann5

  • 1Department of Pediatric Oncology and Hematology, Charité - Universitätsmedizin Berlin, Berlin, Germany.

Pediatric Blood & Cancer
|December 19, 2019
PubMed

Insights

Optimizing donor selection for pediatric hematopoietic stem cell transplantation (HSCT) using HLA-DPB1 and PIRCHE matching is crucial. High PIRCHE scores significantly increase graft-versus-host disease (GvHD) risk in children undergoing HSCT.

Area of Science:

  • Immunogenetics
  • Pediatric Hematology
  • Transplantation Science

Background:

  • Hematopoietic stem cell transplantation (HSCT) strategies are often adult-focused, yet pediatric HSCT differs in disease spectrum and complication risks like graft-versus-host disease (GvHD).
  • Optimizing donor selection is critical for improving outcomes in pediatric HSCT.
  • Understanding genetic factors influencing HSCT success in children is essential.

Purpose of the Study:

  • To evaluate HLA-DPB1 and Predicted Indirectly ReCognizable HLA-Epitope (PIRCHE) matching for donor selection in pediatric HSCT.
  • To minimize GvHD and other complications in children undergoing HSCT.
  • To assess the impact of HLA-DPB1 and PIRCHE matching on HSCT outcomes in a pediatric cohort.

Main Methods:

  • Analysis of HLA-DPB1 and PIRCHE matching in a pediatric cohort undergoing HSCT between 2014 and 2016 in Berlin.
  • Evaluation of the association between HLA-DPB1 mismatches and GvHD or relapse incidence.
  • Assessment of PIRCHE-I scores in relation to GvHD risk, considering HLA-DPB1 matching.

Main Results:

  • Nonpermissive HLA-DPB1 mismatches did not increase GvHD but were linked to higher relapse rates.
  • High PIRCHE-I scores significantly correlated with increased GvHD risk in pediatric HSCT recipients from matched unrelated donors.
  • The association between high PIRCHE scores and GvHD risk remained significant even after incorporating HLA-DPB1 matching.

Conclusions:

  • Implementing PIRCHE typing in donor selection for pediatric HSCT may improve outcomes.
  • PIRCHE-based donor selection could particularly benefit pediatric patients with nonmalignant diseases.
  • Further validation of PIRCHE-based donor selection in larger, multi-center pediatric cohorts is recommended.
Abstract

Keywords:
HLA-DPB1HLA-typingPIRCHEdonor selectionnonmalignant diseasespediatric stem cell transplantation

More Related Videos

Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies11:59

Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies

Published on: September 6, 2017

7.7K
Using Quantitative Real-time PCR to Determine Donor Cell Engraftment in a Competitive Murine Bone Marrow Transplantation Model10:11

Using Quantitative Real-time PCR to Determine Donor Cell Engraftment in a Competitive Murine Bone Marrow Transplantation Model

Published on: March 7, 2013

15.8K

Related Concept Videos

Bone Marrow Sampling and Transplants01:22

Bone Marrow Sampling and Transplants

Bone marrow transplant is a potential cure for several diseases, including cancer and specific genetic disorders. Notably, this procedure is applicable for patients suffering from aplastic anemia, certain types of leukemia, severe combined immunodeficiency disease (SCID), Hodgkin's disease, non-Hodgkin's lymphoma, multiple myeloma, thalassemia, sickle-cell disease, and certain cancers.
The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy...
780
Tissue Transplantation01:24

Tissue Transplantation

Tissue transplantation is a significant medical procedure involving the transfer of cells, tissues, or organs from a donor to a recipient, with the primary aim of restoring lost functions. This procedure is crucial in treating a broad spectrum of diseases, including kidney diseases, liver failure, heart disease, and certain types of cancers.
The Biology of Tissue Transplantation
The biology of tissue transplantation hinges on the Major Histocompatibility Complex (MHC) molecules. These molecules...
831
Kidney Transplant I: Introduction01:28

Kidney Transplant I: Introduction

A kidney transplant is a surgical approach that involves replacing a non-functioning kidney with a healthy one from a donor. This procedure is often a treatment option for end-stage renal disease (ESRD) patients. The method requires careful recipient selection, including evaluating various medical and psychosocial factors. These criteria vary between transplant centers but generally include assessments of the patient's overall health, adherence to medical recommendations, and lifestyle...
284