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Updated: Jan 1, 2026
Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
Published on: April 8, 2016
Expression of stromal elements of prostatic adenocarcinoma in different gleason scores
Clarice Fraga Esteves Maciel Osorio1, Waldemar Silva Costa2, Carla Braga Mano Gallo3
1Fellow PhD degree, Postgraduate Program in Physiopathology and Surgical Sciences, Urogenital Research Unit, Universidade do Estado do Rio de Janeiro (UERJ), Brazil. Conception and design of the study; acquisition, analysis and interpretation of data; technical procedures; histological examinations; statistics analysis; manuscript preparation and writing; final approval.
Purpose:
To quantify and compare the expression of stromal elements in prostate adenocarcinoma of different Gleason scores with non-tumor area (control).
Methods:
We obtained 132 specimens from samples of prostate peripheral and transition zone. We analyzed the following elements of the extracellular matrix: collagen fibers, elastic system, smooth muscle fibers and blood vessels. The tumor area and non-tumor area (control) of the TMA (tissue microarray) were photographed and analyzed using the ImageJ software.
Results:
The comparison between the tumor area and the non-tumor area showed significant differences between stromal prostate elements. There was an increase of collagen fibers in the tumor area, mainly in Gleason 7. Elastic system fibers showed similar result, also from the Gleason 7. Blood vessels showed a significant increase occurred in all analyzed groups. The muscle fibers exhibited a different behavior, with a decrease in relation to the tumor area.
Conclusions:
There is a significant difference between the extracellular matrix in prostate cancer compared to the non-tumor area (control) especially in Gleason 7. Important modifications of the prostatic stromal elements strongly correlate with different Gleason scores and can contribute to predict the pathological staging of prostate cancer.
