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Updated: Jan 1, 2026

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
Published on: June 23, 2022
Identification of a Small Probe That Can Be Conjugated to Proteins by Proximity Labeling
Weiping Sun1,2,3, Yinbo Huo1,2,3, Yuxuan Mei1
1School of Life Science and Technology , ShanghaiTech University , Shanghai , China.
Abstract:
Proximity labeling has been used to study protein-protein interactions and can also be used as a protein labeling tool. We developed a novel 14-amino acid peptide substrate for the proximity-labeling enzyme PafA. The N terminus of the peptide can be modified with biotin or fluorophores, which allows various chemical moieties to be ligated to the target protein. We used PafA-mediated peptide labeling to label antibodies with a biotin tag without affecting the antigen binding capacity of the antibody. Similar strategies can be used for other types of protein labeling in the future.
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