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Characterization of a human liver reference material fit for proteomics applications
W Clay Davis1, Lisa E Kilpatrick2, Debra L Ellisor3
1National Institute of Standards and Technology, Material Measurement Laboratory, Chemical Sciences Division, Charleston, South Carolina, 29412, USA. clay.davis@nist.gov.
Scientific Data
|December 20, 2019
Summary
The National Institute of Standards and Technology (NIST) developed a new human liver reference material for proteomics. This material supports data harmonization and benchmarking across laboratories and techniques.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Proteomics
Background:
- The National Institute of Standards and Technology (NIST) is developing new reference materials for proteomics.
- Economical, qualitative reference materials are needed for comparisons, benchmarking, and harmonization in proteomics.
Purpose of the Study:
- To describe a large dataset from shotgun proteomic analysis of RM 8461 Human Liver for Proteomics.
- To demonstrate the utility of this reference material for assessing sample-to-data workflows and enabling inter-laboratory harmonization.
Main Methods:
- Shotgun proteomic analysis of RM 8461 Human Liver.
- Consensus identification using multiple search engines and sample preparation techniques.
Main Results:
- The human liver reference material (RM 8461) is homogeneous and fit-for-purpose.
- The data generated can be used to assess complete sample-to-data workflows.
- The material facilitates benchmarking and harmonization between different laboratories and techniques.
Conclusions:
- RM 8461 Human Liver for Proteomics is a valuable resource for the proteomics community.
- This reference material supports the standardization and quality control of proteomics measurements.
- The associated dataset (PXD013608) is publicly available for further research and validation.
