Purine and pyrimidine dinucleoside polyphosphates differentially affect the phenylpropanoid pathway in Vitis vinifera...

It is known that the concentration of dinucleoside polyphosphates (Np_nN's) in cells increases under stress and that adverse environmental factors induce biosynthesis of phenylpropanoids, which protect the plant against stress. Previously, we showed that purine Np_nN's such as Ap₃A and Ap₄A induce both the activity of enzymes of the phenylpropanoid pathway and the expression of relevant genes in Arabidopsis seedlings. Moreover, we showed that Ap₃A induced stilbene biosynthesis in Vitis vinifera cv. Monastrell suspension cultured cells. Data presented in this paper show that pyrimidine-containing Np_nN's also modify the biosynthesis of stilbenes, affecting the transcript level of genes encoding key enzymes of the phenylpropanoid pathway and of these, Up₄U caused the most effective accumulation of trans-resveratrol in the culture media. Similar effect was caused by Ap₃A and Gp₃G. Other pyrimidine Np_nN's, such as Cp₃C, Cp₄C, and Ap₄C, strongly inhibited the biosynthesis of stilbenes, but markedly (6- to 8-fold) induced the expression of the cinnamoyl-CoA reductase gene that controls lignin biosynthesis. Purine counterparts also clearly induced biosynthesis of trans-resveratrol and trans-piceid, but only slightly induced the expression of genes involved in lignin biosynthesis. In cells, Up₃U caused a greater accumulation of trans-resveratrol and trans-piceid than did Up₄U. Each of the Np_nN's studied induced expression of the gene encoding the resveratrol transporter VvABCG44, which operates within the Vitis vinifera cell membrane. AMP, GMP, UMP, and CMP, potential products of Np_nN degradation, did not affect the accumulation of stilbenes. The results obtained strongly support that Np_nN's play a role as signaling molecules in plants.