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Related Experiment Videos

Viral myb oncogene encodes a sequence-specific DNA-binding activity.

H Biedenkapp1, U Borgmeyer, A E Sippel

  • 1Zentrum für Molekulare Biologie der Universität Heidelberg, FRG.

Nature
|October 27, 1988
PubMed
Summary

The v-MYB oncogene binds DNA sequences, specifically pyAACG/TG. This finding suggests MYB proteins are sequence-specific DNA-binding factors crucial for gene regulation.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Oncology

Background:

  • The retroviral oncogene v-myb and its cellular counterpart c-myb encode nuclear DNA-binding proteins.
  • MYB genes are conserved across diverse species, including vertebrates, insects (Drosophila melanogaster), and plants (Zea mays).

Purpose of the Study:

  • To investigate the DNA-binding specificity of the v-MYB protein.
  • To understand the role of MYB/DNA interactions in gene regulation.

Main Methods:

  • Analysis of the DNA-binding domain localization within the v-MYB protein.
  • Experimental determination of the specific nucleotide sequence recognized by v-MYB.

Main Results:

  • The DNA-binding domain of v-MYB is located in a highly conserved amino-terminal region.

Related Experiment Videos

  • v-MYB specifically recognizes and binds to the nucleotide sequence pyAACG/TG.

    • Conclusions:

    • v-MYB functions as a sequence-specific DNA-binding factor.
    • MYB proteins are likely involved in regulating gene expression through specific DNA interactions.