Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Yolk sac development in dogs: from morphology to functional aspects.

Animal reproduction·2026
Same author

Elastographic and ultrasound findings of the dog`s kidneys exposed to cigarette smoke.

Brazilian journal of veterinary medicine·2025
Same author

DWORF expression is reduced in a large animal model of Duchenne muscular dystrophy.

Disease models & mechanisms·2025
Same author

Circulatory CCL2 distinguishes Duchenne muscular dystrophy dogs.

Disease models & mechanisms·2025
Same author

Adeno-Associated Virus 8 and 9 Myofibre Type/Size Tropism Profiling Reveals Therapeutic Effect of Microdystrophin in Canines.

Journal of cachexia, sarcopenia and muscle·2025
Same author

Genetic reduction of skeletal muscle glycogen synthase 1 abundance reveals that the refeeding-induced reversal of elevated insulin-stimulated glucose uptake after exercise is not attributable to achieving a high muscle glycogen concentration.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2024

Related Experiment Video

Updated: Jan 1, 2026

Diagnostic Ultrasound Imaging of Mouse Diaphragm Function
07:12

Diagnostic Ultrasound Imaging of Mouse Diaphragm Function

Published on: April 21, 2014

16.5K

An improved method for studying mouse diaphragm function.

Chady H Hakim1,2, Thais B Lessa1,3, Gregory J Jenkins1,4

  • 1Department of Molecular Microbiology and Immunology, School of Medicine, The University of Missouri, Columbia, MO, 65212, USA.

Scientific Reports
|December 21, 2019
PubMed
Summary
This summary is machine-generated.

A new clamp method accurately measures diaphragm muscle contractility in mice. This technique provides reliable force data, crucial for studying respiratory and neuromuscular diseases.

More Related Videos

Functional and Morphological Assessment of Diaphragm Innervation by Phrenic Motor Neurons
09:43

Functional and Morphological Assessment of Diaphragm Innervation by Phrenic Motor Neurons

Published on: May 25, 2015

18.1K
Repeated Measurement of Respiratory Muscle Activity and Ventilation in Mouse Models of Neuromuscular Disease
09:24

Repeated Measurement of Respiratory Muscle Activity and Ventilation in Mouse Models of Neuromuscular Disease

Published on: April 17, 2017

13.5K

Related Experiment Videos

Last Updated: Jan 1, 2026

Diagnostic Ultrasound Imaging of Mouse Diaphragm Function
07:12

Diagnostic Ultrasound Imaging of Mouse Diaphragm Function

Published on: April 21, 2014

16.5K
Functional and Morphological Assessment of Diaphragm Innervation by Phrenic Motor Neurons
09:43

Functional and Morphological Assessment of Diaphragm Innervation by Phrenic Motor Neurons

Published on: May 25, 2015

18.1K
Repeated Measurement of Respiratory Muscle Activity and Ventilation in Mouse Models of Neuromuscular Disease
09:24

Repeated Measurement of Respiratory Muscle Activity and Ventilation in Mouse Models of Neuromuscular Disease

Published on: April 17, 2017

13.5K

Area of Science:

  • Physiology
  • Biomedical Engineering
  • Respiratory Medicine

Background:

  • Diaphragm muscle dysfunction is a key factor in respiratory and neuromuscular diseases.
  • Accurate quantification of diaphragm function in mouse models is vital for preclinical research.
  • Existing methods for measuring diaphragm contractility have limitations.

Purpose of the Study:

  • To develop and validate a novel diaphragm clamp method for precise muscle force measurement.
  • To compare the efficacy of the new clamp method against the traditional suture method.
  • To provide a detailed protocol for clamp fabrication and application.

Main Methods:

  • Engineered durable diaphragm clamps.
  • Developed a step-by-step protocol for clamp fabrication and diaphragm strip mounting.
  • Compared diaphragm force measurements using both clamp and suture methods in the same mouse.
  • Validated the clamp method in the mdx mouse model of Duchenne muscular dystrophy.

Main Results:

  • The clamp method demonstrated superior maintenance of physiological muscle fiber orientation.
  • Diaphragm force measurements were significantly higher using the clamp method compared to the suture method.
  • The clamp method proved reliable and consistent in the mdx mouse model.

Conclusions:

  • The developed diaphragm clamp method offers a reliable and accurate approach for assessing diaphragm contractility in mice.
  • This standardized protocol facilitates consistent and reproducible diaphragm function assays.
  • The clamp method is a valuable tool for preclinical studies of respiratory and neuromuscular disorders.