Aloe Vera/Collagen Mixture Induces Integrin α1β1 and PECAM-1 Genes Expression in Human Adipose-Derived Stem Cells

Area of Science:

• Biomaterials Science
• Tissue Engineering
• Stem Cell Biology

Background:

• Collagen is a primary component of the extracellular matrix (ECM) and a vital biomaterial in tissue engineering.
• Aloe vera possesses therapeutic properties that may enhance collagen's functionality.

Purpose of the Study:

• To investigate the impact of an Aloe vera/collagen blend on human adipose-derived stem cells (hASCs).
• To assess effects on cell viability, attachment, and angiogenic potential by examining integrin α1β1 and PECAM-1 gene expression.

Main Methods:

• hASCs were cultured in vitro with control, collagen gel, Aloe vera gel, or Aloe vera/collagen blend.
• Cell viability was measured using the MTT assay.
• Gene expression of integrin α1β1 and PECAM-1 was quantified via real-time RT-PCR.

Main Results:

• The Aloe vera/collagen blend maintained and improved hASC viability compared to controls.
• Gene expression of integrin α1β1 and PECAM-1 was significantly upregulated in the Aloe vera/collagen group.
• This suggests enhanced angiogenic potential and cell-matrix interactions.

Conclusions:

• Aloe vera/collagen blend positively influences hASC behavior for tissue engineering applications.
• The blend promotes cell viability and upregulates key genes associated with angiogenesis and cell adhesion.
• Aloe vera can be a valuable adjunct to collagen biomaterials.