Electrospun Decellularized Skeletal Muscle Tissue/Polycaprolactone/Polyaniline as a Potential Scaffold for Muscle...

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Tissue Engineering

Background:

• Skeletal muscle has limited self-healing capacity after extensive damage.
• Loss of muscle cells and extracellular matrix hinders regeneration.
• Tissue engineering strategies are crucial for restoring muscle function.

Purpose of the Study:

• To develop and characterize a novel electrospun scaffold for skeletal muscle tissue engineering.
• To investigate the impact of polyaniline on scaffold properties.
• To evaluate the biological and myogenic effects of the scaffold on human Wharton's jelly mesenchymal stem cells (hWJ-MSCs).

Main Methods:

• Electrospinning of decellularized skeletal muscle tissue (DSM)/polycaprolactone (PCL)/polyaniline (PANi) composite.
• Characterization of structural and physicochemical properties.
• In vitro assessment of hWJ-MSC behavior and myogenic differentiation.

Main Results:

• The DSM/PCL/PANi scaffold exhibited conductive fibrous properties with favorable physical and chemical characteristics.
• The scaffold demonstrated biocompatibility with hWJ-MSCs, promoting cell adhesion and proliferation.
• Significant upregulation of myogenic markers (MyoD, Myogenin, MHC) was observed in hWJ-MSCs cultured on the scaffold.

Conclusions:

• The electrospun DSM/PCL/PANi scaffold is a promising biomaterial for muscle tissue engineering.
• Its biocompatibility and ability to promote stem cell differentiation are beneficial for skeletal muscle regeneration.
• This composite scaffold represents a viable approach for developing advanced biological scaffolds.