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The Effect of Bacterial Peptide p28 on Viability and Apoptosis Status of P53-null HeLa Cells

Haniyeh Abuei1, Abbas Behzad-Behbahani2, Fatemeh Faghihi3

  • 1Department of Medical Laboratory Sciences, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.

Advanced Pharmaceutical Bulletin
|December 21, 2019
PubMed

Insights

The bacterial peptide p28 did not significantly affect the viability or apoptosis of p53-null HeLa cancer cells. Its anti-cancer efficacy appears dependent on the presence of the tumor-suppressor protein p53.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cancer Research

Background:

  • Cancer remains a global health challenge, driving the search for novel therapeutics.
  • The bacterial peptide p28 exhibits anti-cancer properties by inhibiting the degradation of the tumor-suppressor protein p53.
  • This study investigates p28's effectiveness in p53-null cancer cells.

Purpose of the Study:

  • To evaluate the anti-proliferative and apoptotic effects of the bacterial peptide p28.
  • To determine if p28's anti-cancer activity is dependent on the presence of p53.
  • To assess p28's potential as a therapeutic agent for p53-negative cancers.

Main Methods:

  • The p28 gene was amplified from Pseudomonas aeruginosa and expressed in E. coli.
  • Peptide purification was performed using Ni-NTA chromatography.
  • Cell viability and apoptosis were assessed using MTT and PE Annexin V Flowcytometry assays in HeLa and HEK-293 cells.

Main Results:

  • Purified p28 concentration was determined to be 0.05 mg/mL.
  • MTT assays showed minimal impact on HeLa cell viability (84% at 2.5 µM p28).
  • Flow cytometry revealed no significant increase in apoptosis in p53-null HeLa cells treated with p28.

Conclusions:

  • p28 demonstrated no statistically significant effect on the viability or apoptosis of p53-null HeLa cells.
  • The anti-cancer efficacy of p28 is contingent upon the presence of the p53 protein.
  • p28 is likely unsuitable for treating cancers lacking p53 expression.
Keywords:
ApoptosisNull HeLa cellsViabilityp28p53

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