Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Genetic Markers Remain Detectable in Genetically Engineered Microbes Biocontained with a CRISPR Kill Switch.

Environmental science & technology·2026

Same author

Engineering microbial consortia for mixed plastic upcycling.

Nature communications·2025

Same author

Developing an alternative medium for in-space biomanufacturing.

Nature communications·2025

Same author

Influence of Environmental Conditions on the Escape Rates of Biocontained Genetically Engineered Microbes.

Environmental science & technology·2024

Same author

Manipulating the molecular specificity of transcriptional biosensors for tryptophan metabolites and analogs.

Cell reports. Physical science·2024

Same author

Engineering E. coli strains using antibiotic-resistance-gene-free plasmids.

Cell reports methods·2023

Same journal

LaeA Orchestrates Iron-Heme Supply and P450 Catalytic Efficiency for Enhanced Echinocandin B Biosynthesis in Aspergillus nidulans.

Biotechnology journal·2026

Same journal

Emerging Bioengineering Technologies in Female Reproduction: Preclinical Advances, Translational Challenges, and Future Outlook.

Biotechnology journal·2026

Same journal

Multi-Enzyme Cascade Reaction of Crude Enzyme Strategy for the Economical and Efficient Bioconversion of Rebaudioside A to Rebaudioside M.

Biotechnology journal·2026

Same journal

Metabolic and Synthetic Biology Strategies for Enhancing Single-Cell Protein Production in Saccharomyces cerevisiae.

Biotechnology journal·2026

Same journal

Severe Restriction of Glucose Import Enhances Recombinant Protein Production in Escherichia coli.

Biotechnology journal·2026

Same journal

Butyrate-Producing Bacteria in Intestinal Disease Therapy: Potential and Challenges.

Biotechnology journal·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 1, 2026

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

Biosensing in Smart Engineered Probiotics.

Austin G Rottinghaus¹, Matthew B Amrofell¹, Tae Seok Moon^1,2

¹Department of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, St. Louis, MO, 63130, USA.

Biotechnology Journal

|December 21, 2019

Summary

This summary is machine-generated.

Engineered microbes with biosensors offer new ways to diagnose and treat diseases. These "smart" microbes can sense signals in the body, report on health, and deliver therapies, advancing medical applications.

Keywords:

biocontainment biosensing diagnostic probiotic therapeutic

More Related Videos

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Published on: November 20, 2013

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Published on: October 8, 2021

Related Experiment Videos

Last Updated: Jan 1, 2026

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Published on: November 20, 2013

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Published on: October 8, 2021

Area of Science:

Synthetic biology
Microbial engineering
Biotechnology

Background:

Engineered microbes present novel solutions for diagnostics and therapeutics.
Genetic tools enable engineering of probiotic and commensal microbes.
Biosensors allow microbes to detect and respond to biological signals.

Purpose of the Study:

To review the application of biosensing in engineering "smart" microbes.
To discuss in vivo diagnostic, therapeutic, and biocontainment applications.
To identify challenges and future advancements in biosensing for medical use.

Main Methods:

Review of current literature on engineered microbes and biosensing technologies.
Analysis of genetic tools and parts for microbial engineering.
Discussion of in vitro to in vivo transition challenges.

Main Results:

Biosensors enable engineered microbes to act as in vivo diagnostics and therapeutics.
Smart microbes can sense, record, and respond to host signals.
Advancements are needed to bridge the gap between in vitro and in vivo systems.

Conclusions:

Engineered microbes with biosensing capabilities hold significant promise for medical applications.
Overcoming in vivo transition challenges is crucial for clinical translation.
Continued development of microbial engineering technologies will address future medical needs.