Enhanced granulation process, a more effective way of aerobic granular sludge cultivation in pilot-scale application...

Aerobic granular sludge (AGS) could be cultivated from only flocs (called normal granulation (NG) process) or mixture of flocs and crushed AGS (called enhanced granulation (EG) process), which might lead to different system performances such as granulation speed and pollutants removal efficiencies. However, the differences of mechanisms between NG and EG processes at microbial community level are still unknown. In this study, the NG and EG processes were implemented successively in a pilot-scale sequencing batch reactor (SBR) with certain amounts of additional carbon sources. Illumina MiSeq sequencing and quantitative PCR were applied to investigate the dynamics of bacterial communities during NG and EG processes and explore the possible explanations for faster EG process. The results showed that significant distinctions in bacterial diversities and community structures were observed between NG and EG processes. The major contributor to NG process was bacterial communities with 32.04% contribution. While EG process was more dependent on the interactions (73.16% contribution) between the bacterial communities and environmental variables (operational parameters and self-adaptive variable). EG process had higher relative abundances of functional bacteria than NG process. Glycogen accumulating organisms (GAOs) related bacteria with a total relative abundance of maximum 65.43% might be mainly responsible for the faster EG process. This study provided microbial insights for practical application of AGS technology that inoculating crushed AGS might be an effective way to cultivate AGS.