Semen analysis and sperm characteristics of Karan Fries cattle

Karan Fries is an Indian composite breed of cattle with superior milk production with a greater disease resistance than many other cattle. Understanding of the basic sperm and semen characteristics is necessary to develop the artificial insemination and sperm sexing methods. This study was conducted to evaluate the morphological, rheological and biochemical characteristics of Karan Fries (Indian composite breed) bull semen. Semen analysis was conducted using Computer Assisted Sperm Analysis (CASA) and rheological methods. Results from correlation assessments indicated there was a positive correlation among values for sperm velocity variables. Motility of bull sperm movements in a linear path were the important variables evaluated in this study. There was also characterization of the individual spermatozoa using scanning electron microscopy (SEM) and Raman spectroscopy. The sperm head length and width were 8.8 ± 0.8 and 3.9 ± 0.6 μm, respectively, and the approximate flagellum length was 59.4 ± 4.5 μm. There was a difference in the Raman intensity among the individual spermatozoa. Results of this study could be important in designing and developing methods of artificial insemination, cryopreservation of semen and semen sexing.