Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corneal and Endothelial Parameters Following Scleral Lens Wear in Post-Keratoplasty Eyes.

Ophthalmic & physiological optics : the journal of the British College of Ophthalmic Opticians (Optometrists)·2026
Same author

Repeatability of choroidal optical coherence tomography angiography metrics in healthy young adults.

Clinical & experimental optometry·2026
Same author

Short-Term Effects of Optically Induced and Digitally Simulated Myopic Blur on Retinal Perfusion: Insights From Optical Coherence Tomography Angiography.

Investigative ophthalmology & visual science·2026
Same author

Six-Month Changes in Accommodation and Vergence in Juvenile Myopes Wearing Multifocal Soft Contact Lenses.

Eye & contact lens·2026
Same author

Tear Dynamics During Fenestrated Scleral Lens Wear: A Pilot Study.

Ophthalmic & physiological optics : the journal of the British College of Ophthalmic Opticians (Optometrists)·2026
Same author

Crystalline Lens Shape During Accommodation in Children.

Ophthalmic & physiological optics : the journal of the British College of Ophthalmic Opticians (Optometrists)·2026

Related Experiment Video

Updated: Jan 1, 2026

Author Spotlight: Anterior HR-OCT as a Non-Invasive Tool for Characterizing Ocular Surface Squamous Neoplasia
06:15

Author Spotlight: Anterior HR-OCT as a Non-Invasive Tool for Characterizing Ocular Surface Squamous Neoplasia

Published on: August 9, 2024

1.7K

Anterior segment optical coherence tomography scanning protocols and corneal thickness repeatability.

Damien Fisher1, Michael J Collins1, Stephen J Vincent1

  • 1Contact Lens and Visual Optics Laboratory, School of Optometry and Vision Science, Queensland University of Technology, Australia.

Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association
|December 23, 2019
PubMed
Summary

Anterior segment optical coherence tomography (AS-OCT) protocols using 3 lines of 20 B-scans provide repeatable corneal thickness measurements. This method offers reliable data for monitoring subtle corneal changes.

Keywords:
Anterior segment OCTCorneal oedemaCorneal thicknessRepeatability

More Related Videos

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
07:44

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography

Published on: July 24, 2020

3.3K
Doppler Optical Coherence Tomography of Retinal Circulation
10:46

Doppler Optical Coherence Tomography of Retinal Circulation

Published on: September 18, 2012

19.2K

Related Experiment Videos

Last Updated: Jan 1, 2026

Author Spotlight: Anterior HR-OCT as a Non-Invasive Tool for Characterizing Ocular Surface Squamous Neoplasia
06:15

Author Spotlight: Anterior HR-OCT as a Non-Invasive Tool for Characterizing Ocular Surface Squamous Neoplasia

Published on: August 9, 2024

1.7K
In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
07:44

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography

Published on: July 24, 2020

3.3K
Doppler Optical Coherence Tomography of Retinal Circulation
10:46

Doppler Optical Coherence Tomography of Retinal Circulation

Published on: September 18, 2012

19.2K

Area of Science:

  • Ophthalmology
  • Medical Imaging
  • Corneal Science

Background:

  • Accurate corneal thickness measurement is crucial for diagnosing and monitoring various ocular conditions.
  • Anterior segment optical coherence tomography (AS-OCT) is a key technology for in vivo corneal imaging.
  • Optimizing AS-OCT imaging protocols is essential for reliable and repeatable measurements.

Purpose of the Study:

  • To evaluate how different AS-OCT imaging protocols affect the repeatability of corneal thickness measurements.
  • To determine the optimal number of B-scans and line scans for consistent results.
  • To assess intraobserver and intrasession variability in epithelial, stromal, and total corneal thickness.

Main Methods:

  • AS-OCT (Spectralis, Heidelberg) was used to capture images from 15 adult participants.
  • Single line scans with varying B-scan averages (2-100) and volumetric scans (9 lines) were performed.
  • Corneal thickness was measured using single point and averaged central 6 mm methods.

Main Results:

  • Intraobserver repeatability for single line scans was not significantly affected by B-scan averaging.
  • Volumetric scans showed increased scan duration with more line scans but minimal impact on quality.
  • Averaging more than 3 line scans did not significantly improve repeatability for thickness measurements.

Conclusions:

  • AS-OCT volumetric scans with 3 lines (20 B-scans each) provide highly repeatable corneal thickness measurements.
  • The protocol ensures reliable intraobserver (≤ ±3.2 μm) and intrasession (≤ ±3.7 μm) repeatability.
  • This optimized protocol is suitable for monitoring subtle corneal thickness changes.