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Updated: Jan 1, 2026

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
Published on: April 7, 2014
Two improved defocus quantitative phase imaging methods: discussion
Abstract:
Multifilter phase imaging with partially coherent light (MFPI-PC) and phase optical transfer function recovery (POTFR) are two viable defocus-based, two-dimensional quantitative phase imaging (QPI) methods. While both methods use transfer function inversion, MFPI-PC is based on the in-focus intensity derivative, while POTFR is based on the intensity difference between symmetrically defocused images. This paper compares and contrasts MFPI-PC and POTFR. Six disadvantages (five in MFPI-PC and one in POTFR) are identified. Improvement strategies to overcome each of the six shortcomings are identified and implemented, and both methods are shown to be clearly improved. The revised MFPI-PC is shown to be more accurate than the original MFPI-PC and generally more accurate than the revised POTFR. The revised POTFR is shown to be inherently faster than the original POTFR and also slightly faster than the revised MFPI-PC.
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