LC-MS Compatible Antiadsorption Diluent for Peptide Analysis.

Area of Science:

• Analytical Chemistry
• Biochemistry

Background:

• Peptide analysis is hindered by adsorption to plastic and glass, causing sample loss and unreliable data, particularly at low concentrations.
• Existing antiadsorption methods are often incompatible with direct liquid chromatography-mass spectrometry (LC-MS).

Purpose of the Study:

• To develop a novel, LC-MS compatible antiadsorption diluent for peptide analysis.
• To mitigate peptide adsorption to glass consumables.

Main Methods:

• Controlled hydrolysis and precipitation of bovine serum albumin (BSA) to create a novel antiadsorption diluent.
• Evaluation of the diluent's efficacy in reducing peptide adsorption to glass.
• Assessment of the diluent's compatibility with LC-MS, formic acid, and acetonitrile.

Main Results:

• The novel BSA-based diluent significantly decreased the adsorption of specific peptides to glass surfaces.
• The diluent demonstrated compatibility with direct LC-MS analysis.
• The diluent maintained efficacy when used with formic acid and/or acetonitrile.

Conclusions:

• A new BSA-based diluent effectively reduces peptide adsorption, enhancing analytical reliability.
• This diluent offers a practical, LC-MS compatible solution for challenging peptide analyses.