Inhibition of platelet aggregation by an irreversible local anesthetic

Three known local anesthetic agents were examined for their ability to inhibit platelet aggregation induced by adenosine diphosphate or epinephrine. Drug-treated platelet-rich plasma samples were filtered through agarose gel to remove free drug, and the ability of the platelets to aggregate was again determined. One of the local anesthetics contained a "one-armed" nitrogen mustard structure and appeared to produce an irreversible inhibition of aggregation. The other two agents gave a block of aggregation that was reversed upon gel filtration.