Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Methodology and quality assurance practices in performing anti-extractable nuclear antigen (ENA) analysis: an EFLM report on an international survey.

Clinical chemistry and laboratory medicine·2026
Same author

Early Placental Angioactive Response to Maternal SARS-CoV-2 Infection: an Immunohistochemical Study.

Reproductive sciences (Thousand Oaks, Calif.)·2026
Same author

Anti-CENP-A/B reactivity in samples exhibiting the centromere HEp-2 pattern is associated with a lower frequency of interstitial lung disease in limited cutaneous systemic sclerosis patients.

Journal of circulating biomarkers·2025
Same author

The spectrum of nuclear patterns with stained metaphase chromosome plate: morphology nuances, immunological associations, and clinical relevance.

Clinical chemistry and laboratory medicine·2025
Same author

Y12C mutation disrupts IMPDH cytoophidia and alters cancer metabolism.

The FEBS journal·2025
Same author

Evaluation of Heterogeneity in the Coding Region of BRAF, MAP2K1, and MAP2K2 Genes in Primary and Metastatic Melanomas.

Journal of cutaneous pathology·2024

Related Experiment Video

Updated: Jun 23, 2026

Detection and Isolation of Circulating Melanoma Cells using Photoacoustic Flowmetry
09:52

Detection and Isolation of Circulating Melanoma Cells using Photoacoustic Flowmetry

Published on: November 25, 2011

16.3K

IMP dehydrogenase rod/ring structures in acral melanomas.

Gerson D Keppeke1, Denise Barcelos2, Mariana Fernandes2

  • 1Rheumatology Division, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil.

Pigment Cell & Melanoma Research
|December 29, 2019
PubMed
Summary

Acral lentiginous melanoma (ALM) cells exhibit IMPDH-based rods/rings (RR) structures. Detecting these RR structures in biopsies may help diagnose ALM and assess its malignancy.

Keywords:
IMP dehydrogenaseacral lentiginous melanomacutaneous malignantmelanocytic nevimelanomarod/ring structurestissue micro-array

More Related Videos

Combining Reflectance Confocal Microscopy with Optical Coherence Tomography for Noninvasive Diagnosis of Skin Cancers via Image Acquisition
09:37

Combining Reflectance Confocal Microscopy with Optical Coherence Tomography for Noninvasive Diagnosis of Skin Cancers via Image Acquisition

Published on: August 18, 2022

2.8K
Author Spotlight: Recreating Melanoma Complexity with Patient-Derived Organoids for Immunotherapy Evaluation
05:33

Author Spotlight: Recreating Melanoma Complexity with Patient-Derived Organoids for Immunotherapy Evaluation

Published on: September 6, 2024

2.2K

Related Experiment Videos

Last Updated: Jun 23, 2026

Detection and Isolation of Circulating Melanoma Cells using Photoacoustic Flowmetry
09:52

Detection and Isolation of Circulating Melanoma Cells using Photoacoustic Flowmetry

Published on: November 25, 2011

16.3K
Combining Reflectance Confocal Microscopy with Optical Coherence Tomography for Noninvasive Diagnosis of Skin Cancers via Image Acquisition
09:37

Combining Reflectance Confocal Microscopy with Optical Coherence Tomography for Noninvasive Diagnosis of Skin Cancers via Image Acquisition

Published on: August 18, 2022

2.8K
Author Spotlight: Recreating Melanoma Complexity with Patient-Derived Organoids for Immunotherapy Evaluation
05:33

Author Spotlight: Recreating Melanoma Complexity with Patient-Derived Organoids for Immunotherapy Evaluation

Published on: September 6, 2024

2.2K

Area of Science:

  • Oncology
  • Biochemistry
  • Pathology

Background:

  • Acral lentiginous melanoma (ALM) is an aggressive melanoma subtype.
  • The enzyme IMPDH (Inosine Monophosphate Dehydrogenase) forms filamentary structures (rods/rings, RR) linked to high GTP demand in proliferative cells.

Purpose of the Study:

  • To investigate the presence and diagnostic utility of IMPDH-based RR structures in ALM.
  • To determine if RR structures correlate with ALM aggressiveness.

Main Methods:

  • Immunohistochemical analysis of IMPDH2 in 45 ALM and 59 nevi tissue samples.
  • Quantification of RR-positive cells and ROC curve analysis for diagnostic accuracy.
  • Correlation of RR status with histopathological features like Breslow thickness.

Main Results:

  • IMPDH-based RR structures were observed more frequently in ALM than in nevi (80% vs. 15%, p < .001).
  • ROC analysis yielded an AUC of 0.88, with a cutoff of 5.5% RR-positive cells showing 80% sensitivity and 85% specificity for ALM.
  • RR-positive ALM specimens were more likely to have a Breslow thickness > 4.0mm (71% vs. 29%, p = .039).

Conclusions:

  • IMPDH-based RR structures are significantly more prevalent in ALM compared to nevi.
  • Screening for RR structures in biopsies may serve as a valuable diagnostic aid for ALM.
  • The presence of RR structures may indicate a more malignant tumor phenotype in ALM.