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Electrolysis phenomena in electrophoresis.

Tomáš Novotný1, Bohuslav Gaš1

  • 1Charles University, Faculty of Science, Department of Physical and Macromolecular Chemistry, Prague, Czech Republic.

Electrophoresis
|December 29, 2019
PubMed
Summary

This study introduces a theoretical method to predict buffer changes in capillary electrophoresis (CE) caused by electrolysis. The approach helps ensure robust separations by evaluating buffer suitability for repeated experiments.

Keywords:
Background electrolyteCapillary zone electrophoresisContactless conductivity detectionElectrolysis

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Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Electrolysis in electrode vials is unavoidable during capillary electrophoresis (CE) measurements.
  • This electrolysis alters background electrolyte (BGE) composition, negatively impacting separation robustness and quality.
  • Predicting BGE changes is crucial for assessing BGE suitability for reproducible electrophoretic runs.

Purpose of the Study:

  • To develop and validate a theoretical approach for calculating physico-chemical property changes in BGEs due to electrolysis in CE.
  • To compare theoretical predictions with experimental measurements of pH and conductivity changes in BGEs.

Main Methods:

  • A novel theoretical model was developed to calculate BGE property alterations from electrolysis.
  • Experimental validation involved measuring pH and conductivity of BGEs post-CE using microelectrodes and contactless detection.
  • The model's accuracy was tested with a common BGE (weak acid-strong base) and a Good's buffer.

Main Results:

  • The theoretical approach accurately predicted changes in BGE physico-chemical properties.
  • Good's buffers exhibited significantly lower property changes compared to common BGEs after electrophoretic runs.
  • This confirms the theoretical model's validity and highlights the stability of Good's buffers under CE conditions.

Conclusions:

  • The presented theoretical method effectively predicts BGE changes caused by electrolysis in CE.
  • Good's buffers demonstrate superior stability and are more suitable for robust, repeatable CE separations compared to traditional BGEs.
  • This work provides a valuable tool for optimizing CE methods and ensuring reliable analytical results.