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Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
Application of engineered yeast strain fermentation for oligogalacturonides production from pectin-rich waste biomass
Guojun Yang1, Haidong Tan2, Shuguang Li2
1Dalian Engineering Research Center for Carbohydrate Agricultural Preparations, Liaoning Provincial Key Laboratory of Carbohydrates, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100049, China; College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
Abstract:
Citrus wastes disposal is a problem faced by many juice plants due to high disposal costs. However, the citrus peel wastes (CPW) biomass, as bulk bioresources from the agro-industrial waste, is a good source of pectin. Present study aimed to utilize these CPW biomass by engineered yeast strain fermentation with an inexpensive method to produce oligogalacturonides (OGs). The results showed that the engineered yeast strain fermentation can produce significant amounts of OGs with the degree of polymerization ranged from 2 to 7 from the CPW bioresources. Under the optimized conditions using the response surface methodology, the best OGs yield were 26.1%. The present work is the first to use the engineered yeast strain for direct CPW biomass fermentation produced the OGs. We thereby paved a new way to utilize the pectin-rich bioresources.

