Efficient polysaccharides from Crinum asiaticum L.'s structural characterization and anti-tumor effect

In this study, an efficient polysaccharide, named CAL-n (Crinum asiaticum L.-n) was isolated and purified from Crinum asiaticum L for the first time, Mw(molecular weight) of 730,000 Da. CAL-n comprised Rha(rhamnose), Sor(sorbose), Gal(galactose) and Glu(glucosein) the molar ratio of 1:61.6:1.66:4.74. The chemical structure of CAL-n was studied by Infrared spectrum and GC-MS(Gas Chromatography-Mass Spectrometer) analysis. Experimental results reflected, that the backbone of CAL-n comprised (1 → 2), (1 → 6), (1 → 3) beta-pyran glycoside bond, without (1 → 4) beta-pyran glycoside bond. In addition, an MTT assay indicated that the growth of HepG₂ cells was affected by CAL-n, with a concentration dependant ration. The results indicated that CAL-n should by exploration as anti-tumor activities in vivo.