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Anticancer activity and metabolite profiling data of Penicillium janthinellum KTMT5
Kudzanai Ian Tapfuma1, Tendani Edith Sebola2, Nkemdinma Uche-Okereafor2
1DST/NRF Centre of Excellence for Biomedical Tuberculosis Research and SAMRC Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Stellenbosch University, PO Box 19063, Tygerberg, 7505, Cape Town, South Africa.
Abstract:
Fungi are ubiquitous, they proliferate even in environments with toxic pollutants that are otherwise harmful to other eukaryotes. This article presents data of fungi which were isolated from gold mine tailings and identified by DNA sequencing of their inter transcribed spacer regions 1 and 2. Five fungal isolates were identified, among which the crude extract of Penicillium janthinellum KTMT5 was investigated for anticancer activity on A549 (lung carcinoma) and UMG87 (glioblastoma) cell lines. Untargeted metabolite profiling of the crude extract of P. janthinellum KTMT5 was performed using liquid chromatography quadrupole time of flight tandem mass spectrometry (LC-QTOF-MS/MS) and a molecular network generated using the online workflow on the Global Natural Product Social molecular networking (GNPS) website. DNA sequencing showed that all fungal isolates belonged to phylum Ascomycota with the genus Penicillium representing 75% of the fungal isolates. P. janthinellum KTMT5 which was selected for further experiments showed significant anticancer activity against UMG87 cells with a calculated IC50 value of 44.23 μg/mL in the MTS assay, while the real time xCELLigence assay showed dose-dependent anticancer activity at 50 and 100 μg/mL. Metabolite profiling revealed the presence of several known metabolites in the crude extract of P. janthinellum KTMT5 and molecular networking showed the relationships among these metabolites.
Insights
Fungi from gold mine tailings, including Penicillium janthinellum KTMT5, show anticancer potential. This fungus exhibited significant activity against glioblastoma cells, warranting further investigation for novel cancer therapies.
Area of Science:
- Microbiology
- Pharmacology
- Biotechnology
Background:
- Fungi thrive in extreme environments, including toxic mine tailings.
- These environments may harbor unique fungal species with potential biotechnological applications.
Purpose of the Study:
- To isolate and identify fungi from gold mine tailings.
- To investigate the anticancer activity of fungal extracts, specifically Penicillium janthinellum KTMT5.
- To characterize the metabolites present in P. janthinellum KTMT5 extract.
Main Methods:
- Fungal isolation from gold mine tailings.
- DNA sequencing of inter transcribed spacer regions for fungal identification.
- Anticancer activity assays (MTS and xCELLigence) on A549 and UMG87 cell lines.
- Untargeted metabolite profiling using LC-QTOF-MS/MS.
- Molecular networking using GNPS.
Main Results:
- Five fungal isolates were identified, all belonging to the phylum Ascomycota.
- Penicillium janthinellum KTMT5 demonstrated significant anticancer activity against UMG87 (glioblastoma) cells (IC50 = 44.23 μg/mL).
- Dose-dependent anticancer effects were observed in real-time assays at 50 and 100 μg/mL.
- Metabolite profiling identified known compounds, and molecular networking elucidated their relationships.
Conclusions:
- Fungi isolated from gold mine tailings represent a potential source of bioactive compounds.
- Penicillium janthinellum KTMT5 extract shows promising anticancer activity against glioblastoma, meriting further drug development research.
- Metabolomic analysis provides insights into the chemical diversity of this fungal isolate.
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