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Updated: Dec 31, 2025

Employing Pressurized Hot Water Extraction PHWE to Explore Natural Products Chemistry in the Undergraduate Laboratory
Published on: November 7, 2018
Pressurized hot water extraction of hydrosable tannins from Phyllanthus tenellus Roxb
Noor Hidayah Mohd Jusoh1, Atiqah Subki1, Swee Keong Yeap2
11Metabolomics Laboratory, Biotechnology Centre, Malaysian Agriculture Research and Development Institute (MARDI), Serdang, Selangor Malaysia.
Background:
Safety, environmental and economic setbacks are driving industries to find greener approaches to extract bioactive compounds from natural resources. Pressurized hot water extraction (PHWE) is among the solvent free and efficient methods for extracting bioactive compounds.
Experimental:
In this study, the suitability of PHWE for extracting bioactive compounds such as phenolics, hydrolysable tannins and flavonoids from Phyllanthus tenellus was investigated by UPLC-qTOF-MS.
Results:
Solvent properties of water are significantly increased through imposing temperature at 121 °C and pressure at 15 p.s.i. Pressurized hot water extraction obtained 991-folds higher hydrolysable tannins than methanol extraction.
Conclusion:
The extraction yields of hydrolysable tannins with PHWE was almost double of absolute methanol extraction.

