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Published on: June 8, 2016
A polar stationary phase obtained by surface-initiated polymerization of hyperbranched polyglycerol onto silica
Huiliang Geng1, Jie Jing1, Feifang Zhang1
1Engineering Research Center of Pharmaceutical Process Chemistry, East China University of Science and Technology, Shanghai, 200237, China.
Abstract:
A novel polar stationary phase for hydrophilic interaction chromatography (HILIC) is prepared by surface-initiated polymerization of hyperbranched polyglycerol onto silica. Such polymerization offers an easy way to tune the cabon load. The stationary phase showed obvious hydrogen bonding interaction and typical HILIC character, exhibiting nealy-universal separation ability towards polar analytes. Good peak shape and high separation efficiency was observed for model polar analytes, including nucleosides and nucleic bases, aromatic acids, organic bases, vitamins, saccharides, amino acids, e.g. 35,000-58,000 plate counts per meter for five water-soluble vitamins.
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