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A novel gold nanostars-based fluorescent aptasensor for aflatoxin B1 detection
Min Wei1, Fei Zhao1, Yanli Xie1
1College of Food Science and Technology, Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control, Henan University of Technology, Zhengzhou, 450001, PR China.
Talanta
|January 2, 2020
Summary
A novel fluorescent aptasensor using gold nanostars efficiently detects aflatoxin B1 (AFB1). This method offers high sensitivity and selectivity for AFB1 detection in food samples.
Area of Science:
- Nanomaterials Science
- Biosensing Technology
- Analytical Chemistry
Background:
- Aflatoxin B1 (AFB1) poses significant health risks, necessitating sensitive detection methods.
- Existing detection techniques often lack the required sensitivity or speed for real-time monitoring.
- Development of novel sensing platforms is crucial for accurate food safety analysis.
Purpose of the Study:
- To develop a simple, sensitive, and novel fluorescent aptasensor for the detection of aflatoxin B1 (AFB1).
- To utilize gold nanostars (AuNSs) as an efficient fluorescence quenching material for enhanced detection.
- To validate the aptasensor's performance in complex matrices like corn flour.
Main Methods:
- Design of a carboxyfluorescein-labeled hairpin DNA (FAM-labeled HP) that hybridizes with an AFB1 aptamer.
- Modification of AuNSs with the DNA-aptamer complex, utilizing fluorescence resonance energy transfer (FRET) for signal generation.
- Induction of fluorescence quenching upon AFB1 binding to the aptamer, causing conformational changes and hairpin restoration.
Main Results:
- The AuNSs-based aptasensor demonstrated a superior fluorescence quenching effect compared to gold nanoparticles (AuNPs).
- A linear relationship was observed between fluorescence intensity changes and AFB1 concentrations from 0.1 ng/mL to 10 ng/mL.
- The limit of detection (LOD) was determined to be 21.3 pg/mL, with good selectivity and recovery rates (92%-112%) in spiked corn flour samples.
Conclusions:
- The proposed fluorescent aptasensor based on AuNSs offers a highly sensitive and selective method for AFB1 detection.
- The novel application of AuNSs as a fluorescence quencher enhances the aptasensor's performance.
- This aptasensor shows great potential for practical application in food safety monitoring.
