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Updated: Dec 31, 2025
Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources
Published on: September 3, 2009
Methods for Target Enrichment Sequencing via Probe Capture in Legumes
Ze Peng1, Dev Paudel1, Liping Wang1
1Agronomy Department, University of Florida, Gainesville, FL, USA.
Abstract:
Target enrichment sequencing (TES) is a powerful approach to deep-sequencing the exome or genomic regions of interest with great depth. Although successfully and widely adopted in many plant species, TES is currently applied for genotyping of only a couple legume species. Here we describe an in-solution probe capture based method for application of TES in legumes. The topics cover probe design, library preparation, probe hybridization, as well as bioinformatic analysis for evaluation of target capture efficiency and identifying single nucleotide polymorphisms using generated sequencing data.
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