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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Updated: Dec 31, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
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CRISPR/Cas9-Based Gene Editing in Soybean.

Aili Bao1, Lam-Son Phan Tran2,3, Dong Cao4

  • 1Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, China.

Methods in Molecular Biology (Clifton, N.J.)
|January 2, 2020
PubMed
Summary
This summary is machine-generated.

This study details a robust CRISPR/Cas9 gene editing method for soybean. The described protocols facilitate efficient soybean transformation and regeneration, advancing functional genomics and crop improvement.

Keywords:
Agrobacterium-mediated soybean transformationCRISPR/Cas9Cotyledonary nodeGene editingSoybeanTissue culture

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Area of Science:

  • Agricultural Biotechnology
  • Molecular Biology
  • Plant Science

Background:

  • CRISPR/Cas9 gene editing is crucial for crop functional genomics and breeding.
  • Soybean research has utilized CRISPR/Cas9, but a standardized detailed protocol is lacking.

Purpose of the Study:

  • To provide a detailed methodology for CRISPR/Cas9 gene editing in soybean.
  • To establish efficient Agrobacterium-mediated transformation and regeneration protocols for soybean.

Main Methods:

  • Construction of CRISPR/Cas9 plasmids for soybean gene editing.
  • Modified Agrobacterium-mediated transformation protocols.
  • Regeneration of soybean from cotyledonary node explants.

Main Results:

  • Successful construction of CRISPR/Cas9 plasmids tailored for soybean.
  • Optimized protocols for Agrobacterium-mediated soybean transformation.
  • Efficient regeneration of soybean plants from explants carrying Cas9/sgRNA transgenes.

Conclusions:

  • This work presents a comprehensive CRISPR/Cas9 gene editing method for soybean.
  • The established protocols will accelerate soybean functional genomics research and breeding programs.