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Related Concept Videos

RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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Predicting Amputation using Local Circulating Mononuclear Progenitor Cells in Angioplasty-treated Patients with Critical Limb Ischemia
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Noncoding RNAs in Critical Limb Ischemia.

Daniel Pérez-Cremades1,2, Henry S Cheng1, Mark W Feinberg1

  • 1From the Cardiovascular Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA (D.P.-C., H.S.C., M.W.F.).

Arteriosclerosis, Thrombosis, and Vascular Biology
|January 3, 2020
PubMed
Summary
This summary is machine-generated.

Noncoding RNAs, including microRNAs and long noncoding RNAs, play key roles in critical limb ischemia (CLI) development and progression. Understanding these ncRNAs offers new avenues for preventing, diagnosing, and treating CLI.

Keywords:
diabetes mellitusinflammationmicroRNAsperfusionperipheral artery disease

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Area of Science:

  • Vascular Biology
  • Molecular Medicine
  • Genetics

Background:

  • Peripheral artery disease (PAD) affects over 200 million globally, potentially progressing to critical limb ischemia (CLI).
  • CLI increases risks of amputation and cardiovascular mortality, with current treatments like therapeutic angiogenesis showing limited success.
  • Angiogenic resistance, particularly in conditions like diabetes mellitus, is a significant challenge in managing CLI.

Purpose of the Study:

  • To review the role of noncoding RNAs (ncRNAs) in the pathophysiology of critical limb ischemia.
  • To highlight ncRNAs as functional mediators and potential biomarkers in CLI.
  • To explore how understanding ncRNAs can lead to novel therapeutic strategies for CLI.

Main Methods:

  • Literature review focusing on noncoding RNAs (microRNAs, long noncoding RNAs) and their involvement in CLI.
  • Analysis of ncRNA regulation in key CLI-related processes like angiogenesis, hypoxia, inflammation, and stem cell function.
  • Examination of ncRNA roles in the context of comorbidities such as diabetes mellitus.

Main Results:

  • Noncoding RNAs are crucial regulators of gene expression impacting multiple CLI pathophysiologies.
  • Specific ncRNAs function as mediators in processes like angiogenesis and inflammation relevant to CLI.
  • ncRNAs show potential as biomarkers for CLI diagnosis and prognosis.

Conclusions:

  • Noncoding RNAs are integral to the complex pathophysiology of critical limb ischemia.
  • Targeting ncRNAs presents promising opportunities for developing new diagnostic and therapeutic approaches for CLI.
  • Further research into ncRNAs could significantly advance the management of this debilitating vascular disease.