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Related Experiment Video

Updated: Dec 31, 2025

Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons
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How Peptides Dissociate in Plasmonic Hot Spots.

Jacek Szczerbiński1, Jonas B Metternich1, Guillaume Goubert1

  • 1Department of Chemistry and Applied Biosciences, Laboratory of Organic Chemistry, ETH Zurich, 8093, Zurich, Switzerland.

Small (Weinheim an Der Bergstrasse, Germany)
|January 3, 2020
PubMed
Summary
This summary is machine-generated.

Plasmon-driven chemistry breaks strong bonds in biomolecules like peptides using visible light. This process, observed with tip-enhanced Raman spectroscopy (TERS), mimics electron transfer dissociation (ETD) in mass spectrometry.

Keywords:
amide I bandelectron transfer dissociationhot electronspeptidesplasmon-driven photocatalysistip-enhanced Raman spectroscopy

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Area of Science:

  • Chemistry
  • Biochemistry
  • Materials Science

Background:

  • Plasmon-induced hot carriers can break strong chemical bonds using visible light, a phenomenon previously shown for small molecules.
  • The application of plasmon-driven photochemistry to complex biomolecules like proteins and peptides remained largely unexplored.

Purpose of the Study:

  • To extend the scope of plasmon-driven photochemistry to biomolecules.
  • To investigate the reactivity of proteins and peptides within plasmonic hot spots.
  • To elucidate the mechanism of plasmon-induced bond dissociation in peptides.

Main Methods:

  • Utilizing tip-enhanced Raman spectroscopy (TERS) to both initiate and monitor photochemical reactions.
  • Analyzing changes in TER spectra, specifically the amide I band, to detect peptide backbone bond dissociation.
  • Comparing the observed fragmentation pathway with known electron-induced dissociation mechanisms.

Main Results:

  • Peptide backbone bonds were successfully dissociated within plasmonic hot spots under visible light irradiation.
  • Disappearance of the amide I band in TER spectra indicated peptide fragmentation.
  • The fragmentation pathway was identified as nonthermal activation via dissociative capture of hot electrons.

Conclusions:

  • Plasmon-driven photochemistry can effectively induce dissociation of peptide backbone bonds.
  • The observed mechanism shares similarities with electron transfer dissociation (ETD) in mass spectrometry.
  • This analogy provides a potential design principle for future plasmon-induced biomolecular reactions.