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Updated: Dec 31, 2025

Optimized Griess Reaction for UV-Vis and Naked-eye Determination of Anti-malarial Primaquine
Published on: October 11, 2019
Rational modulation of coumarin-hemicyanine platform based on OH substitution for higher selective detection of
Jin-Shuai Lan1, Li Liu, Rui-Feng Zeng
1Experiment Center of Teaching & Learning, Shanghai University of Traditional Chinese Medicine, Shanghai, China. dingyue-2001@hotmail.com.
Abstract:
To monitor delicate changes of biological HOCl in vivo, a new probe (OH-substituted coumarin-hemicyanine, probe 2) was synthesized for NIR and ratiometric HOCl detection. Selectivity studies indicated that the electron-donating group (OH) substituted on the indolium moiety enhanced the selectivity to detect HOCl. With HOCl, the probe showed a ratiometric fluorescence (I500/I650) with a low detection limit (49.1 nM) and a rapid response (within 2 min). In addition, probe 2 was successfully applied to visualize exogenous and endogenous HOCl in living cells and animals and exhibited a perfect mitochondria target ability. This probe has been further studied as a potential and powerful tool to probe HOCl in arthritis models.
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