Related Experiment Video
Updated: Dec 31, 2025
07:01
3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography
Published on: October 24, 2019
10.2K
A partial-dithering strategy for edge-illumination x-ray phase-contrast tomography enabled by a joint reconstruction
Yujia Chen1, Charlotte K Hagen2, Alessandro Olivo2
1Department of Biomedical Engineering, Washington University in St Louis, Campus Box 1097, One Brookings Drive, St Louis, MO, 63130, United States of America.
Physics in Medicine and Biology
|January 3, 2020
Summary
Edge-illumination x-ray phase-contrast tomography (EIXPCT) can achieve high resolution using dithering. A new partial-dithering strategy reduces acquisition time while maintaining comparable spatial resolution to full dithering.
Area of Science:
- Medical Imaging
- X-ray Tomography
- Materials Science
Background:
- Edge-illumination x-ray phase-contrast tomography (EIXPCT) estimates refractive index distribution.
- Image resolution is limited by mask period but improved by dithering.
- Dithering increases data acquisition time and radiation dose.
Purpose of the Study:
- Propose a novel partial-dithering strategy for EIXPCT data acquisition.
- Reduce data acquisition time and radiation dose.
- Maintain high spatial resolution.
Main Methods:
- Implemented dithering at a subset of tomographic view angles.
- Utilized a joint reconstruction method for data analysis.
- Explored the effect of dithering parameters on image resolution.
Main Results:
- Partial dithering achieved spatial resolution comparable to full dithering.
- Acquisition time was substantially decreased compared to full dithering.
- The strategy offers a balance between resolution and efficiency.
Conclusions:
- Partial dithering is an effective strategy for EIXPCT.
- Reduces acquisition time and radiation dose without compromising resolution.
- Enables more flexible data-acquisition designs.
Related Concept Videos
01:07Electron Microscope Tomography and Single-particle Reconstruction
2.8K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.8K
01:10Computed Tomography
7.9K
Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
7.9K
01:26Phase Contrast and Differential Interference Contrast Microscopy
11.9K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
11.9K
01:27Imaging Studies III: Computed Tomography
229
DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
229
01:24X-ray Imaging
9.6K
German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with...
9.6K